A man who said he was Timmothy Pitzen is not the boy who went missing in 2011, according to DNA tests, the Federal Bureau of Investigation said Thursday.
Authorities also released a video clip of 6-year-old Timmothy playing a make-believe guitar, hoping that the boy's disappearance would remain in the public eye.
But FBI officials in Kentucky and OH said DNA tests conducted at the Cincinnati Children's Hospital showed that he was neither a teenager, nor Timmothy. "Unfortunately, that day will not be today", Federal Bureau of Investigation spokesman Timothy Beam in Louisville said in a statement.
"We never stopped looking for him". The witness also said you could "see the fear on him and how nervous he was".
The FBI's Louisville and Cincinnati offices are coordinating with police from Newport, Ky., Cincinnati and Aurora, Ill., to determine if the boy is Timmothy Pitzen who has been missing since May 2011 when he was 6 years old, officials from the FBI's Louisville field office said.
He was said to have crossed state lines from OH into Kentucky where he was found around 7:30 a.m. Wednesday and where he told witnesses had been on the run for two hours.
The boy escaped from a Red Roof Inn, a U.S. discount hotel, where he says he was being held by the two kidnappers, according to an OH police report filed on Wednesday. The Chicago Tribune reported that detectives from suburban Aurora, Illinois, left Wednesday for Ohio.
The teen told police he escaped his captors and proceeded to run across a bridge into Kentucky. Authorities are now working to confirm the identity of the 14-year-old boy, who claims he has been held captive over the past seven years.
At the time, police searched for Pitzen in Illinois, Wisconsin and Iowa.
The men drove a Ford SUV with Wisconsin plates, according to the police.
Aurora police Sgt. Bill Rowley called the person's claim "a disappointment" and that this was another time the family had their "hope raised".
She said Timmothy knew his home address and grandmother's phone number when he disappeared. But they're hoping to have something later Thursday. "He lived in IL that's what he told me and he just was passed around to people that he didn't know", she said.
Linda Ramirez, who lives nearby and knew the family, said she was "pretty excited" but didn't "want to have false hopes".
Meanwhile, the mother-son pair traveled to the Brookfield Zoo, KeyLime Cove water park and a Kalahari Resorts in the Wisconsin Dells.
At the time of Timmothy's disappearance, police said that the boy's mother may have dropped him off with a friend, noting that the boy's auto seat and Spider-Man backpack were gone.
According to a report from the Medina Post, Rini was arrested on June 22, 2015 for making false alarms involving a law enforcement agency.
She left behind notes saying Timmothy was safe but claimed he would never be found again.
Over the years, family members have said the mother had a history of depression, and her marriage was failing.