Spacey was arraigned on a charge of felony indecent assault and battery during a hearing Monday at Nantucket District Court.
The 59-year-old actor flew in for the proceedings via a private plane and appeared to be smiling as he entered the courthouse around 10:45 a.m. local time.
Spacey must stay away from the young man who accused him of groping him at a MA bar in 2016 and his family, the judge ordered.
The Boston Globe reported a not-guilty plea was entered on the two-time Oscar winner's behalf during the hearing in the USA state of MA.
A pre-trial hearing has been set for 4 March.
The judge additionally ordered Spacey to have no contact with the man accusing him of assault, and requested that six months of the accuser's phone data be preserved, The Associated Press reports.
Separately, in September, the Los Angeles County District Attorney's office said Mr Spacey would not be prosecuted over an accusation of sexual assault that allegedly took place in 1992.
It's the first criminal case brought against Spacey after several sexual misconduct allegations crippled his career in 2017. According to the criminal complaint, an 18-year-old busboy at Club Car told Spacey that he was 23 and befriended the actor, known for his roles in the film "The Usual Suspects" and the Netflix series "House of Cards". His lawyer is claiming these records are proof that Spacey did not assault the victim in a Nantucket bar. The victim claims that Spacey fondled his penis.
He said the two were standing in a crowd by a piano when Spacey unzipped the teen's trousers and groped his genitals for about three minutes. Spacey tried to get out of appearing at his arraignment, saying his presence would "amplify the negative publicity already generated" by the case.
"Of course, some believed everything and have just been waiting with bated breath to hear me confess at all", Spacey says in the video, which is titled "Let Me Be Frank".
In 2017 the House of Cards actor announced for the first time he was living "as a gay man" after he was accused of inappropriate behaviour.
"By reporting the sexual assault, my client is a determined and encouraging voice for those victims not yet ready to report being sexually assaulted", said lawyer Mitchell Garabedian, who has represented hundreds of clergy sexual abuse victims.
Spacey became embroiled in controversy in October 2017 when actor Anthony Rapp accused him of trying to seduce him in 1986 when Rapp was 14.