Real Madrid took the last contest between the two, winning 2-1 thanks to goals from Karim Benzema and Marcelo.
An unmarked Benzema notched his second goal in the 37th minute with a redirected header assisted by Gareth Bale to give Real a 3-0 lead and Benzema returned the favour minutes later when he flicked on a cross for Bale to sweep home a volley.
Madrid were dominate throughout and Toni Kroos added an impressive fifth after the interval when he chipped goalkeeper from inside the area. Real Madrid can retain its top spot in the Group C standings with a victory, while Plzen will be eliminated with a loss and if AS Roma beats CSKA Moscow in the group's other game.
"It was an important night for us", Casemiro said.
Benzema, who has made 429 appearances for the club in all competitions, is the seventh player to score at least 200 times for Madrid.
The hosts were more lively after the break and managed a few shots on goal but could not find the quality they needed in the final third to chip away at Real's lead.
Santiago Solari has won both of his games as Real Madrid manager since taking over from Julen Lopetegui. The Czech team tied CSKA Moscow on September 19, but then suffered a blowout loss to Roma on October 2, falling 5-0, before defeat at the Bernabeu.